Mary Mathew has academic degree in MBBS (Bachelor of Medicine, Bachelor of Surgery), Kasturba Medical College, Manipal (1984–1988); DCH (Diploma in Child Health), Kasturba Medical College, Manipal (1990–1991); MD (Doctor of Medicine) in Pathology, Kasturba Medical College, Manipal (1994–1997); PGDMLE (Postgraduate Diploma in Medical Law and Ethics), National Law School of India University (NLSIU), Bengaluru (2012); FIIOPM (Fellow of the International Institute of Organisational Psychological Medicine).